Characterization of Ubiquitin/Proteasome-Dependent Regulation of Hap2/3/4/5 Complex In Saccharomyces cerevisiae
نویسنده
چکیده
......................................................................................................................................... v Introduction ................................................................................................................................... 1 Saccharomyces cerevisiae as a model system .......................................................................... 1 The Metabolism of a Facultative Aerobe ................................................................................ 2 The Hap2/3/4/5 Complex .......................................................................................................... 8 Mitochondrial Biogenesis ....................................................................................................... 13 The Ubiquitin-Protease System ............................................................................................. 14 Materials and Methods ............................................................................................................... 18 Results .......................................................................................................................................... 23 Preliminary Findings from Liu Lab ...................................................................................... 23 Goals of Research .................................................................................................................... 23 Research Results ..................................................................................................................... 24 Discussion .................................................................................................................................... 33 Conclusion ................................................................................................................................... 38 Bibliography ................................................................................................................................ 39
منابع مشابه
The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae.
The regulation of cellular membrane dynamics is crucial for maintaining proper cell growth and division. The Cdc48-Npl4-Ufd1 complex is required for several regulated membrane-associated processes as part of the ubiquitin-proteasome system, including ER-associated degradation and the control of lipid composition in yeast. In this study we report the results of a genetic screen in Saccharomyces ...
متن کاملBiting the hand that feeds: Rpn4-dependent feedback regulation of proteasome function.
The 26S proteasome of eukaryotic cells mediates ubiquitin-dependent as well as ubiquitin-independent degradation of proteins in many regulatory processes as well as in protein quality control. The proteasome itself is a dynamic complex with varying compositions and interaction partners. Studies in Saccharomyces cerevisiae have revealed that expression of proteasome subunit genes is coordinately...
متن کاملCatabolite degradation of fructose-1,6-bisphosphatase in the yeast Saccharomyces cerevisiae: a genome-wide screen identifies eight novel GID genes and indicates the existence of two degradation pathways.
Metabolic adaptation of Saccharomyces cerevisiae cells from a nonfermentable carbon source to glucose induces selective, rapid breakdown of the gluconeogenetic key enzyme fructose-1,6-bisphosphatase (FBPase), a process called catabolite degradation. Herein, we identify eight novel GID genes required for proteasome-dependent catabolite degradation of FBPase. Four yeast proteins contain the CTLH ...
متن کاملPurification of Saccharomyces cerevisiae eIF4E/eIF4G/Pab1p Complex with Capped mRNA
Protein synthesis is one of the most complex cellular processes, involving numerous translation components that interact in multiple sequential steps. The most complex stage in protein synthesis is the initiation process. The basal set of factors required for translation initiation has been determined, and biochemical, genetic, and structural studies are now beginning to reveal details of their...
متن کاملTranscriptional regulation of the squalene synthase gene (ERG9) in the yeast Saccharomyces cerevisiae.
The ergosterol biosynthetic pathway is a specific branch of the mevalonate pathway. Since the cells requirement for sterols is greater than for isoprenoids, sterol biosynthesis must be regulated independently of isoprenoid biosynthesis. In this study we explored the transcriptional regulation of squalene synthase (ERG9) in Saccharomyces cerevisiae, the first enzyme dedicated to the synthesis of...
متن کامل